2. Signaling Pathways
  3. PROTAC
  4. Ligands for E3 Ligase
  5. DCAF1 Isoform

DCAF1

DCAF1 (Ddb1-Cullin4-associated factor 1) serves as a substrate receptor for both CRL4 and EDVP E3 ubiquitin ligases, regulating protein degradation in multiple cellular contexts[1][2]. Mechanistically, DCAF1 recruits specific targets through its WD40 domain, mediating ubiquitination of substrates such as UNG2, PP2A-A, and transcriptional regulators ZIP and sZIP[3][4][5][6]. DCAF1 controls T-cell activation, growth, and proliferation through p53-dependent and independent pathways, and is essential for immune homeostasis and T-cell responses during viral infections[7][8]. In hepatocellular carcinoma models, DCAF1 interacts with PARD3 to activate Akt signaling, promoting tumor progression and metastasis, highlighting its oncogenic potential[9]. Compared with related isoforms, DCAF1 contains a unique LisH-mediated oligomerization region, enabling dimerization of the CRL4-DCAF1 complex, which enhances its E3 ligase activity[3]. In reproductive biology, DCAF1 mediates oocyte meiotic maturation via poly-ubiquitination of PP2A-A, demonstrating substrate-specific roles in developmental pathways[5]. DCAF1 is also a target for HIV-1 Vpr, which hijacks the DCAF1-containing CRL4 complex to induce G2 cell cycle arrest and degrade host restriction factors, illustrating its viral exploitation[10][11][12][13][14]. Recent chemical biology studies have identified small-molecule ligands and PROTACs targeting DCAF1, enabling controlled protein degradation and functional interrogation in cancer and immune cells[15][16][17][12][2].

References:

DCAF1 Related Products (4):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-158301
    MY-1B 2929308-79-0 99.94%
    MY-1B is a covalent inhibitor of the RNA Methyltransferase NSUN2 (IC50: 1.3 μM). MY-1B stereoselectively ligands active-site cysteine residues (C271) of NSUN2. MY-1B can stereoselectively and covalently bind to PSME1, disrupting the proteasome regulatory complex and downregulating the presentation of specific MHC-I subtypes.
    MY-1B
  • HY-169147
    MY-11B 2929223-25-4
    MY-11B is a DCAF1 ligand. MY-11B shows reactivity with DCAF1_C1113. MY-11B blockes YT41R and YT47R-mediated degradation of HA-FKBP12. MY-11B can be used in the synthesis of PROTACs.
    MY-11B
  • HY-169152
    DCAF1 ligand 1 3036941-01-9
    DCAF1 ligand 1 is a DCAF1 ligand, which can be used for the synthesis of PROTACs, such as PROTAC BRD4-DCAF1 degrader-1 (HY-169152).
    DCAF1 ligand 1
  • HY-182974
    DCAF1 binder 3 3033533-52-4
    DCAF1 binder 3 is a DCAF1 binder and an E3 ligase ligand. DCAF1 binder 3 can be used to synthesize PROTAC SAMHD1 Degrader-1 (HY-182970).
    DCAF1 binder 3